Wednesday, 3 January 2007

How Bacteria Can Escape Destruction: Scientists Discover Mechanism Used To Pump Out Drugs

In a new study published online in the open access journal PLoS Biology, Gaby Sennhauser, Marcus Gruetter, and colleagues use structural biology techniques to probe the molecular mechanisms of the major drug efflux pump in E. coli AcrB.
NaN
Bacterial resistance to antibiotics is a major challenge for the current treatment of infectious diseases. One way bacteria can escape destruction is by pumping out administered drugs through specific transporter proteins that span the cell membrane, such as AcrB.
Making use of designer proteins that bind to and stabilize proteins of interest, the researchers were able to obtain better resolution structural data for the AcrB complex. After selecting for designed ankyrin repeat proteins (DARPins) that inhibit this pump, Sennhauser and colleagues solved the crystal structure of the DARPin inhibitor in complex with AcrB. They were able to confirm that the AcrB pump is split into three subunits, each of which exhibit distinctly different conformations.
Each subunit has a differently shaped substrate transport channel; these variable channels provide unique snapshots of the different phases employed by AcrB during transport of a substrate. The structure also offers an explanation for how substrate export is structurally coupled to simultaneous proton import--thus significantly improving our understanding of the mechanism of AcrB. This is the first report of the selection and co-crystallization of a DARPin with a membrane protein, which demonstrates not only DARPins' potential as inhibitors, but also as tools for the structural investigation of integral membrane proteins.
Citation: Sennhauser G, Amstutz P, Briand C, Storchenegger O, Gruetter MG (2007) Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors. PLoS Biol 5(1): e7. doi:10.1371/journal.pbio.0050007.
Note: This story has been adapted from a news release issued by Public Library of Science.
via Science Daily

7 comments:

Anonymous said...

Then the bacteria have developemd mechanism to escape destruction.
Good information.

Thanks
MicrobiologyNewbie

LINKSHARE LEADS said...

Thanks for your blog URL submission at protein crystallization

Anonymous said...

Good post. I learn something new and challenging on
websites I stumbleupon on a daily basis. It's always helpful to read articles from other writers and use something from their web sites.

my website ... frankrijk

Anonymous said...

I needed to thank you for this great read!! I absolutely enjoyed every little bit of it.

I've got you book marked to look at new things you post…

Feel free to visit my web site - homepage

Anonymous said...

I seriously love your website.. Very nice colors & theme.
Did you create this website yourself? Please reply back as I'm planning to create my own personal blog and want to learn where you got this from or just what the theme is called. Appreciate it!

Feel free to surf to my web blog - huis huren frankrijk

Anonymous said...

If you are going for most excellent contents like I do, just go to see this site
every day since it gives feature contents, thanks

My weblog ... luxe vakantiehuizen []

Adams Kevin said...

I ADAMS KEVIN, a representative Aiico Insurance plc, we trust and respect for individual differences in day out a loan. We will provide 2% of the loan's interest rate. If you are interested in this business contact us by e-mail: (adams.credi@gmail.com) now transfer their loan documents issued properly. Do you need a loan to set up business or school if you are very welcome to Aiico Insurance plc. You can also contact us by e-mail: (adams.credi@gmail.com). We first week can request a balance transfer.

DO YOU NEED LOAN FOR PERSONAL BUSINESS? IF YOU CONTACT YOUR EMAIL ABOVE TO PROCEED WITH YOUR LOAN TRANSFER IMMEDIATELY OK.

Google is equally powerful here. You search the web using me!